Is human artificial chromosome used in genome mapping ?
Artificial chromosomes are DNA molecules with a known structure that are manufactured in vitro from specified ingredients that exhibit natural chromosome-like features. Artificial chromosomes were initially developed in budding yeast and have already been used in a large variety of yeast genetics applications. Vectors based on the human artificial chromosome (HAC) provide a potential technology for transferring and producing full-length human genes of any size. Bacteriophage constraints such as restricted cloning capacity, lack of copy number control, and insertional mutagenesis induced by integration into host chromosomes are all addressed by HACs. Transgene silencing has been linked to the random integration of traditional gene delivery vectors like as viruses, plasmids, P1 phage-derived artificial chromosomes, bacterial artificial chromosomes, and yeast artificial chromosomes. Additionally, integrated viral sequences can cause cancer by activating oncogenes at the inse...